Mouse pancreatic alpha amylase (AMY2A)ELISA kit

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€335.00
SKU:
CSB-EL001689MO
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Product Details                            

Target Name

pancreatic alpha amylase (AMY2A)                      
Alternative Names Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit  
Abbreviation AMY2A                            
Uniprot No. P00688                            
Species Mus musculus (Mouse)                        
Sample Types serum, plasma, cell culture supernates, tissue homogenates                  
Detection Range 9.38 mU/mL-600 mU/mL                        
Sensitivity 2.3 mU/mL                          
Assay Time 1-5h                            
Sample Volume 50-100ul                            
Detection Wavelength 450 nm                            
Research Area Metabolism                          
Assay Principle quantitative                          
Measurement Sandwich                            
Precision Intra-assay Precision (Precision within an assay): CV%<8%                  
  Three samples of known concentration were tested twenty times on one plate to assess.              
  Inter-assay Precision (Precision between assays): CV%<10%                  
  Three samples of known concentration were tested in twenty assays to assess.                
                               
Linearity To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
                    Sample   Serum(n=4)    
  1:01                 Average %   97      
                    Range %   92-105      
  1:02                 Average %   92      
                    Range %   88-96      
  1:04                 Average %   86      
                    Range %   80-92      
  1:08                 Average %   89      
                    Range %   84-97      
Recovery The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
                               
  Sample Type               Average % Recovery Range      
  Serum (n=5)               91   87-97      
  EDTA plasma (n=4)               101   94-107      
                               
                               
Typical Data These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.      
                               
  mU/ml OD1 OD2 Average Corrected                    
  600 2.680 2.714 2.697 2.563                    
  300 2.164 2.235 2.200 2.066                    
  150 1.467 1.421 1.444 1.310                    
  75 0.759 0.811 0.785 0.651                    
  37.5 0.533 0.564 0.549 0.415                    
  18.75 0.317 0.326 0.322 0.188                    
  9.38 0.227 0.241 0.234 0.100                    
  0 0.128 0.139 0.134                      
Troubleshooting ELISA kit FAQs                          
and FAQs                              
Storage Store at 2-8°C. Please refer to protocol.                      
Lead Time 3-5 working days                          
 

Citations

Cashew (Anacardium occidentale L.) Nuts Modulate the Nrf2 and NLRP3 Pathways in Pancreas and Lung after Induction of Acute Pancreatitis by Cerulein M Cordaro,Antioxidants,2020

TSG-6 secreted by human adipose tissue-derived mesenchymal stem cells ameliorates severe acute pancreatitis via ER stress downregulation in mice Qiang Li.et al,Stem Cell Research & Therapy,2018Potential Effects of Calcium Binding Protein S100A12 on Severity Evaluation and Curative Effect of Severe Acute Pancreatitis Yinchu Z.et al,Inflammation,2015   

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Target Background

Subcellular Location

Secreted, extracellular space            Protein FamiliesGlycosyl hydrolase 13 family            Database LinksKEGG: mmu:100043684             STRING: 10090.ENSMUSP00000137025            UniGene: Mm.275426