Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Mouse pancreatic alpha amylase (AMY2A)ELISA kit

JopLink Cusabio

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€335.00

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Description

Product Details
Target Name	pancreatic alpha amylase (AMY2A)
Alternative Names	Amy2 ELISA Kit; Amy2a ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
Abbreviation	AMY2A
Uniprot No.	P00688
Species	Mus musculus (Mouse)
Sample Types	serum, plasma, cell culture supernates, tissue homogenates
Detection Range	9.38 mU/mL-600 mU/mL
Sensitivity	2.3 mU/mL
Assay Time	1-5h
Sample Volume	50-100ul
Detection Wavelength	450 nm
Research Area	Metabolism
Assay Principle	quantitative
Measurement	Sandwich
Precision	Intra-assay Precision (Precision within an assay): CV%<8%
	Three samples of known concentration were tested twenty times on one plate to assess.
	Inter-assay Precision (Precision between assays): CV%<10%
	Three samples of known concentration were tested in twenty assays to assess.

Linearity	To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
						Sample	Serum(n=4)
	1:01					Average %	97
						Range %	92-105
	1:02					Average %	92
						Range %	88-96
	1:04					Average %	86
						Range %	80-92
	1:08					Average %	89
						Range %	84-97
Recovery	The recovery of mouse AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

	Sample Type					Average % Recovery	Range
	Serum (n=5)					91	87-97
	EDTA plasma (n=4)					101	94-107


Typical Data	These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

	mU/ml	OD1	OD2	Average	Corrected
	600	2.680	2.714	2.697	2.563
	300	2.164	2.235	2.200	2.066
	150	1.467	1.421	1.444	1.310
	75	0.759	0.811	0.785	0.651
	37.5	0.533	0.564	0.549	0.415
	18.75	0.317	0.326	0.322	0.188
	9.38	0.227	0.241	0.234	0.100
	0	0.128	0.139	0.134
Troubleshooting	ELISA kit FAQs
and FAQs
Storage	Store at 2-8°C. Please refer to protocol.
Lead Time	3-5 working days

Citations

Cashew (Anacardium occidentale L.) Nuts Modulate the Nrf2 and NLRP3 Pathways in Pancreas and Lung after Induction of Acute Pancreatitis by Cerulein M Cordaro,Antioxidants,2020

TSG-6 secreted by human adipose tissue-derived mesenchymal stem cells ameliorates severe acute pancreatitis via ER stress downregulation in mice Qiang Li.et al,Stem Cell Research & Therapy,2018Potential Effects of Calcium Binding Protein S100A12 on Severity Evaluation and Curative Effect of Severe Acute Pancreatitis Yinchu Z.et al,Inflammation,2015