Mono-methyl-Histone H3.1（K36）Antibody | CSB-RA010418A36me1HU

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  • Mono-methyl-Histone H3.1（K36）Antibody
  • Western Blot<br /> Positive WB detected in：HepG2 whole cell lysate，SH-SY5Y whole cell lysate，Rat brain tissue<br /> All lanes：Mono-methyl-Histone H3.1（K36）antibody at 0.6µg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 15 KDa<br /> Observed band size: 15 KDa<br />
  • Immunocytochemistry analysis of CSB-RA010418A36me1HU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-RA010418A36me1HU at 1:37.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
£298.40 - £444.00

Description

Mono-methyl-Histone H3.1（K36）Antibody | CSB-RA010418A36me1HU | Cusabio

Mono-methyl-Histone H3.1（K36）Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant Antibody

Target Names: HIST1H3A

Aliases: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 1F4

Uniport ID: P68431

Modified: Epigenetics and Nuclear Signaling

species: Human

Species Reactivity: Human

Immunogen: A synthesized peptide

Tested Applications: ELISA, WB, ICC, IF; Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:30-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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