Mono-methyl-H2AFZ (K4) Antibody | CSB-PA010100OA04me1HU

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CSB-PA010100OA04me1HU
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3 to 7 Working Days
  • Mono-methyl-H2AFZ (K4) Antibody
  • Immunofluorescence staining of Hela cells with CSB-PA010100OA04me1HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA010100OA04me1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • Immunocytochemistry analysis of CSB-PA010100OA04me1HU diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
€327.50 - €514.70

Description

Mono-methyl-H2AFZ (K4) Antibody | CSB-PA010100OA04me1HU | Cusabio

Mono-methyl-H2AFZ (K4) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: H2AFZ

Aliases: Histone H2A.Z (H2A/z), H2AFZ, H2AZ

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P0C0S5

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Mono-methyl-Lys (4) derived from Human Histone H2A.Z

Immunogen Species: Human

Applications: ELISA, ICC, IF, ChIP

Tested Applications: ELISA, ICC, IF, ChIP; Recommended dilution: ICC:1:1-1:10, IF:1:1-1:10

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: ICC:1:1-1:10

Dilution Ratio3: IF:1:1-1:10

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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