MGMT Antibody | CSB-RA966553A0HU

(No reviews yet) Write a Review
SKU:
CSB-RA966553A0HU
Availability:
3 to 7 Working Days
  • MGMT Antibody
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate<br />
 All lanes: MGMT antibody at 1:1500<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • IHC image of CSB-RA966553A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
€373.00 - €555.00

Description

MGMT Antibody | CSB-RA966553A0HU | Cusabio

MGMT Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: MGMT

Aliases: Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase), MGMT

Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 10G7

Uniport ID: P16455

Modified: Epigenetics and Nuclear Signaling

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human MGMT

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

View AllClose

0 Reviews

View AllClose