LGALS3 Antibody | CSB-RA970289A0HU

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SKU:
CSB-RA970289A0HU
Availability:
3 to 7 Working Days
  • LGALS3 Antibody
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Colo320 whole cell lysate, HT29 whole cell lysate<br />
 All lanes: Galectin 3 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 27 kDa<br />
 Observed band size: 31 kDa<br />
  • IHC image of CSB-RA970289A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of MCF7 Cells with CSB-RA970289A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating LGALS3 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA970289A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA970289A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
$447.60 - $666.00

Description

LGALS3 Antibody | CSB-RA970289A0HU | Cusabio

LGALS3 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: LGALS3

Aliases: Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen), LGALS3, MAC2

Background: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 4,00E+01

Uniport ID: P17931

Modified: Neuroscience; Cell biology

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human Galectin 3

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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