lacZ Monoclonal Antibody | CSB-MA009476A0m

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CSB-MA009476A0m
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3 to 7 Working Days
  • lacZ Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng) <br />
 All lanes: lacZ antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
 Predicted band size: 117 kDa<br />
 Observed band size: 130 kDa<br />
  • Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4&deg;C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) . The image on the right is the 293T cells transfected without lacZ antibody
  • Immunoprecipitating lacZ in 293T transfected whole cell lysate <br />
 Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate.
 Lane 2: CSB-MA009476A0m (2&micro;l) + 293T transfected whole cell lysate (500&micro;g) 
 Lane 3: 293T transfected whole cell lysate (20&micro;g) <br />
 For western blotting, the blot was detected with CSB-MA009476A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000<br />
  • Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4&deg;C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4&deg;C.
€373.00 - €555.00

Description

lacZ Monoclonal Antibody | CSB-MA009476A0m | Cusabio

lacZ Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Monoclonal Antibody

Target Name: lacZ

Aliases: Beta-galactosidase (Beta-gal) (Lactase) , lacZ

Relevance: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 3B7D5

Uniport ID: P00722

Alternatives To SCBT:

Host Species: Mouse

Species Reactivity: Escherichia coli

Immunogen: Recombinant Escherichia coli Beta-galactosidase protein

Immunogen Species: Escherichia coli

Applications: ELISA, WB, IF, IP, FC

Tested Applications: ELISA, WB, IF, IP, FC; Recommended dilution: WB:1:1000-1:64000, IF:1:50-1:200, IP:1ul-4ul, FC:1:100-1:300

Purification Method: >95%, Protein A purified

Dilution Ratio1: WB:1:1000-1:64000

Dilution Ratio2: IF:1:50-1:200

Dilution Ratio3: IP:1ul-4ul

Dilution Ratio4: FC:1:100-1:300

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Tags & Cell Markers

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