Cusabio Polyclonal Antibodies
L Antibody | CSB-PA806333LA01VBE
- 3 to 7 Working Days
L Antibody | CSB-PA806333LA01VBE | Cusabio
L Antibody is Available at Gentaur Genprice with the fastest delivery.
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Product Type: Polyclonal Antibody
Target Names: L
Aliases: RNA-directed RNA polymerase L (Protein L) (Large structural protein) (Replicase) (Transcriptase) [Includes: RNA-directed RNA polymerase (EC 18.104.22.168) ; mRNA (guanine-N (7) -) -methyltransferase (EC 22.214.171.124) ; GDP polyribonucleotidyltransferase (EC 126.96.36.199) ; Cap-specific mRNA (nucleoside-2'-O-) -methyltransferase 2 (EC 188.8.131.526) ], L
Background: RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N) . The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Uniport ID: Q8B0H0
Host Species: Rabbit
Species Reactivity: Vesicular stomatitis Indiana virus
Immunogen: Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L protein (598-784AA)
Immunogen Species: Vesicular stomatitis Indiana virus
Applications: ELISA, WB
Tested Applications: ELISA, WB; Recommended dilution: WB:1:500-1:5000
Purification Method: >95%, Protein G purified
Dilution Ratio1: ELISA:1:2000-1:10000
Dilution Ratio2: WB:1:500-1:5000
Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Initial Research Areas: N/A
Research Areas: N A