KTN1 Antibody | CSB-PA773030LA01HU

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SKU:
CSB-PA773030LA01HU
Availability:
3 to 7 Working Days
  • KTN1 Antibody
  • Western Blot<br />
 Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate<br />
 All lanes: KTN1 antibody at 3.4µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 157, 150, 151, 153 kDa<br />
 Observed band size: 157 kDa<br />
  • IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA773030LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunoprecipitating KTN1 in A549 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA773030LA01HU in A549 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA773030LA01HU (6µg) + A549 whole cell lysate (1mg) <br />
 Lane 3: A549 whole cell lysate (20µg) <br />
£252.64 - £390.96

Description

KTN1 Antibody | CSB-PA773030LA01HU | Cusabio

KTN1 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: KTN1

Aliases: Kinectin (CG-1 antigen) (Kinesin receptor), KTN1, CG1 KIAA0004

Background: Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q86UP2

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Kinectin protein (978-1116AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction

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