ILK Antibody | CSB-RA963626A0HU

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CSB-RA963626A0HU
Availability:
3 to 7 Working Days
  • ILK Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue<br />
 All lanes: ILK antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 52, 45, 37 kDa<br />
 Observed band size: 51 kDa<br />
  • Immunofluorescence staining of MCF7 Cells with CSB-RA963626A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • Immunoprecipitating ILK in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA963626A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA963626A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
$447.60 - $666.00

Description

ILK Antibody | CSB-RA963626A0HU | Cusabio

ILK Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: ILK

Aliases: Integrin-linked protein kinase (EC 2.7.11.1) (59 kDa serine/threonine-protein kinase) (ILK-1) (ILK-2) (p59ILK), ILK, ILK1 ILK2

Background: Receptor-proximal protein kinase regulating integrin-mediated signal transduction (PubMed:8538749, PubMed:9736715). May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (PubMed:8538749, PubMed:9736715).

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 6A5

Uniport ID: Q13418

Modified: Signal transduction

species: Homo sapiens (Human)

Species Reactivity: Human, Mouse

Immunogen: A synthesized peptide derived from human ILK

Tested Applications: ELISA, WB, IF, IP; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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