β-hydroxybutyryl-HIST1H3A (K9) Antibody | CSB-PA010418OA09bhbHU

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CSB-PA010418OA09bhbHU
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  • β-hydroxybutyryl-HIST1H3A (K9) Antibody
  • Western Blot<br /> Positive WB detected in: 293 whole cell lysate (treated with 30mM sodium butyrate for 4h) <br /> All lanes: HIST1H3A antibody at 1µg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 16 kDa<br /> Observed band size: 16 kDa<br />
  • Immunocytochemistry analysis of CSB-PA010418OA09bhbHU diluted at 1:15 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 72h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium 3-hydroxybutyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA09bhbHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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Description

β-hydroxybutyryl-HIST1H3A (K9) Antibody | CSB-PA010418OA09bhbHU | Cusabio

β-hydroxybutyryl-HIST1H3A (K9) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H3A

Aliases: Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P68431

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of β-hydroxybutyryl-Lys (9) derived from Human Histone H3.1

Immunogen Species: Human

Applications: ELISA, WB, ICC, ChIP

Tested Applications: ELISA, WB, ICC, ChIP; Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: ICC:1:10-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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