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HSPA8 Monoclonal Antibody | CSB-MA010829A1m

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CSB-MA010829A1m
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  • HSPA8 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate<br />
 All lanes HSPA8 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 1/50000 dilution<br />
 Predicted band size: 70~75 KDa<br />
 Observed band size: 70~75 KDa<br />
 Exposure time: 5min
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg<br />
 All lanes: HSPA8 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 1/50000 dilution<br />
 Predicted band size: 70~75 KDa<br />
 Observed band size: 70~75 KDa<br />
 Exposure time: 5min
  • Western Blot<br />
 Positive WB detected in: 20μg hela whole cell lysate<br /> 
 HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 1/50000 dilution<br />
 Predicted band size: 70~75 KDa<br />
 Observed band size: 70~75 KDa<br />
 Exposure time: 5min
  • IHC image of CSB-MA010829A1m diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA010829A1m diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-MA010829A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Immunofluorescence staining of MCF-7 cells with CSB-MA010829A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Immunofluorescence staining of PC-3 cells with CSB-MA010829A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Overlay histogram showing MCF-7 cells stained with CSB-MA010829A1m (red line) . The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1/200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00
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Description

HSPA8 Monoclonal Antibody | CSB-MA010829A1m | Cusabio

HSPA8 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: HSPA8

Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1) , HSPA8, HSC70 HSP73 HSPA10

Relevance: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 6E4D2

Uniport ID: P11142

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human

Immunogen: Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB: 1:5000-1:32000, IHC: 1:100-1:500, IF: 1:100-1:300, FC: 1:100-1:300

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:5000-1:32000

Dilution Ratio2: IHC:1:100-1:500

Dilution Ratio3: IF:1:100-1:300

Dilution Ratio4: FC:1:100-1:300

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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