HIST1H2BB (Ab-16) Antibody | CSB-PA010402OA16nacHU

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CSB-PA010402OA16nacHU
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  • HIST1H2BB (Ab-16) Antibody
  • Western Blot<br />
 Positive WB detected in: Hela cell acid extracts, Jurkat cell acid extracts, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue<br />
 All lanes: HIST1H2BB antibody at 2.5µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 14 kDa<br />
 Observed band size: 14 kDa<br />
  • Immunofluorescent analysis of Hela cells using CSB-PA010402OA16nacHU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (CSB-PA010402OA16nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
£262.00 - £411.76

Description

HIST1H2BB (Ab-16) Antibody | CSB-PA010402OA16nacHU | Cusabio

HIST1H2BB (Ab-16) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H2BB

Aliases: Histone H2B type 1-B (Histone H2B.1) (Histone H2B.f) (H2B/f), HIST1H2BB, H2BFF

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P33778

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Peptide sequence around site of Lys (16) derived from Human Histone H2B type 1-B

Immunogen Species: Human

Applications: ELISA, WB, IF, ChIP

Tested Applications: ELISA, WB, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:200-1:2000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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