HIST1H1E (Ab-25) Antibody | CSB-PA010380PA25nme1HU

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CSB-PA010380PA25nme1HU
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  • HIST1H1E (Ab-25) Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate<br />
 All lanes: HIST1H1E antibody at 1:500<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/40000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100
  • Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
€327.50 - €514.70

Description

HIST1H1E (Ab-25) Antibody | CSB-PA010380PA25nme1HU | Cusabio

HIST1H1E (Ab-25) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H1E

Aliases: Histone H1.4 (Histone H1b) (Histone H1s-4), HIST1H1E, H1F4

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P10412

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Lys (25) derived from Human Histone H1.4

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, ChIP

Tested Applications: ELISA, WB, IHC, IF, ChIP; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:2000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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