GST Monoclonal Antibody | CSB-MA000031M0m
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GST Monoclonal Antibody | CSB-MA000031M0m | Cusabio
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Product Type: Tag/Control Antibodies
Target Name: GST
Aliases: Glutathione S-Transferase, gst, GST tag
Relevance: Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Host Species: Mouse
Species Reactivity: All
Immunogen: Recombinant GST Protein
Applications: ELISA, WB
Tested Applications: ELISA, WB; Recommended dilution:WB:1:500-1:5000
Purification Method: >95%,Protein G purified
Dilution Ratio1: WB:1:500-1:5000
Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.