GPRC6A Antibody | CSB-PA722571LA01HU

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SKU:
CSB-PA722571LA01HU
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3 to 7 Working Days
  • GPRC6A Antibody
  • IHC image of CSB-PA722571LA01HU diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA722571LA01HU diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate<br />
 All lanes: GPRC6A antibody at 4.7µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 105, 85, 97 kDa<br />
 Observed band size: 97 kDa<br />
  • Immunofluorescence staining of HepG2 cells with CSB-PA722571LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
€315.80 - €488.70

Description

GPRC6A Antibody | CSB-PA722571LA01HU | Cusabio

GPRC6A Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: GPRC6A

Aliases: G-protein coupled receptor family C group 6 member A (hGPRC6A) (G-protein coupled receptor GPCR33) (hGPCR33), GPRC6A

Background: Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca (2+) and Mg (2+) . Activated by extracellular calcium and osteocalcin. Seems to act through a G (q) /G (11) and G (i) -coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinate nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalent ions and its responsiveness to anabolic steroids.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q5T6X5

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human G-protein coupled receptor family C group 6 member A protein (403-581AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience;Signal transduction

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