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GFAP Monoclonal Antibody | CSB-MA009369A0m

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CSB-MA009369A0m
Availability:
3 to 7 Working Days
  • GFAP Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: Mouse brain tissue, Rat brain tissue<br />
 All lanes: GFAP antibody at 2.7µg/ml<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 50, 51 kDa<br />
 Observed band size: 50 kDa<br />
  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100
  • Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescent analysis of SH-SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing SH-SY5Y cells stained with CSB-MA009369A0m (red line) . The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10<sup>6</sup>cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00
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Description

GFAP Monoclonal Antibody | CSB-MA009369A0m | Cusabio

GFAP Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: GFAP

Aliases: Glial fibrillary acidic protein, GFAP

Relevance: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Isotype: IgG2b

Conjugate: Non-conjugated

Clone Number: 1C91F1

Uniport ID: P14136

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human GFAP protein (292-432AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:500-1:5000

Dilution Ratio2: IHC:1:50-1:500

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Neuroscience;Tags & Cell Markers;Signal transduction;Stem cells

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