GBF1 Antibody | CSB-PA846077ESR1HU

GBF1 Antibody | CSB-PA846077ESR1HU | Cusabio

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Product Type: Polyclonal Antibody

Target Names: GBF1

Aliases: Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1), GBF1, KIAA0248

Background: Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmatic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530) . Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609) . Has GEF activity towards ARF1 (PubMed:15616190) . Has in vitro GEF activity towards ARF5 (By similarity) . Involved in the processing of PSAP (PubMed:17666033) . Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980) . The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352) . May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782) . In neutrophils, involved in G protein-coupled receptor (GPCR) -mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q92538

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 protein (1-85AA)

Immunogen Species: Human

Applications: ELISA, IHC, IF

Tested Applications: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IHC:1:20-1:200

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction