Western Blot: Positive WB detected in: 15μg hela whole cell lysate GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000 = Secondary<br />
Goat polyclonal to mouse IgG at 1/50000 dilution< Predicted band size: 36 KDa<br />
Observed band size: 36 KDa Exposure time: 5min

Western Blot Positive WB detected in: Hela whole cell lysate at 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg All lanes: GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of HepG2 cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000071M0m in Hela whole cell lysate. Lane 2: CSB-MA000071M0m (1µl) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) For western blotting, the blot was detected with CSB-MA000071M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000

GAPDH Monoclonal Antibody | CSB-MA000071M0m

Cusabio Tag & Control

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€256.00 - €360.00

SKU:: CSB-MA000071M0m
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Description

GAPDH Monoclonal Antibody | CSB-MA000071M0m | Cusabio

GAPDH Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Tag/Control Antibodies

Target Name: GAPDH

Aliases: GAPDH; G3PD; GAPD; MGC88685

Relevance: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1’s nuclear localization signal, and degrades Siah1’s substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer’s disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson’s disease, binds to GAPDH and displays neuroprotective actions.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 14C2F11

Host Species: Mouse

Species Reactivity: Human, Rat, Rabbit

Immunogen: Recombinant Human GAPDH protein (3-335AA)

Applications: ELISA, WB, IHC, IP, IF, FC

Tested Applications: ELISA, WB, IHC, IP, IF, FC; Recommended dilution: WB:1:5000-1:1600000, IHC:1:50-1:500, IF:1:50-1:200, IP:1µl-2µl, FC:1:100-1:300

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:5000-1:1600000

Dilution Ratio2: IHC:1:50-1:500

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: IP:1µl-2µl

Dilution Ratio5: FC:1:100-1:300

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.