GALT Antibody | CSB-PA009226LA01HU

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SKU:
CSB-PA009226LA01HU
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3 to 7 Working Days
  • GALT Antibody
  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA009226LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA009226LA01HU at dilution of 1:100
  • Western Blot<br />
 Positive WB detected in: Rat liver tissue, Mouse kidney tissue<br />
 All lanes: GALT antibody at 3µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 44, 32 kDa<br />
 Observed band size: 44 kDa<br />
  • Immunofluorescent analysis of Hela cells using CSB-PA009226LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Immunoprecipitating GALT in HepG2 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA009226LA01HU in HepG2 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA009226LA01HU (8µg) + HepG2 whole cell lysate (500µg) <br />
 Lane 3: HepG2 whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

GALT Antibody | CSB-PA009226LA01HU | Cusabio

GALT Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: GALT

Aliases: Galactose-1-phosphate uridylyltransferase (Gal-1-P uridylyltransferase) (EC 2.7.7.12) (UDP-glucose--hexose-1-phosphate uridylyltransferase), GALT

Background: Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P07902

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human Galactose-1-phosphate uridylyltransferase protein (1-379AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cancer;Metabolism;Signal transduction

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