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Flag Tag Monoclonal Antibody | CSB-MA000021M0m

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CSB-MA000021M0m
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  • Flag Tag Monoclonal Antibody
  • Western Blot<br /> Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 35 kDa<br /> Observed band size: 35 kDa<br />
  • Western Blot<br /> Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng<br /> All lanes: Flag Tag antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 35 kDa<br /> Observed band size: 35 kDa<br />
  • Western Blot<br /> Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50ng<br /> All lanes: Flag Tag antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to mouse IgG at 1/50000 dilution<br /> Predicted band size: 59.6, 36, 35, 42 kDa<br /> Observed band size: 59.6, 36, 35, 42 kDa<br />
  • Western Blot<br /> Positive WB detected in: Flag Tag fusion protein at 10ng<br /> All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000<br /> Secondary<br /> Goat polyclonal to Mouse IgG at 1/50000 dilution<br /> Predicted band size: 35 kDa<br /> Observed band size: 35 kDa<br />
  • Western Blot<br /> Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng<br /> All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000<br /> Secondary<br /> Goat polyclonal to Mouse IgG at 1/50000 dilution<br /> Predicted band size: 35 kDa<br /> Observed band size: 35 kDa<br />
  • Western Blot<br /> Positive WB detected in: 5 different recombinant proteins with Flag Tag <br /> All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000<br /> Secondary<br /> Goat polyclonal to Mouse IgG at 1/50000 dilution<br />
  • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate <br /> Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate<br /> Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg) <br /> Lane 3: Transfected HEK-293 cell whole cell lysate (20µg) <br /> For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 <br />
  • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate<br /> Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br /> Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)<br /> Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)<br />
  • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.
€256.00 - €360.00
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Description

Flag Tag Monoclonal Antibody | CSB-MA000021M0m | Cusabio

Flag Tag Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Tag/Control Antibodies

Target Name: Flag Tag

Aliases: DDK tag, DYKDDDDK, Flag, Flag Tag

Relevance: Flag-tag, or Flag octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

Isotype: IgG2a

Conjugate: Non-conjugated

Clone Number: 4G5C8

Host Species: Mouse

Species Reactivity:

Immunogen: DYKDDDDK(Flag) synthetic peptide conjugate to KLH

Applications: ELISA, WB, IF, IP

Tested Applications: ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:1000-1:10000

Dilution Ratio2: IF:1:100-1:300

Dilution Ratio3: IP:0.5µl-2µl

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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