EIF4A2 Antibody | CSB-PA00384A0Rb

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CSB-PA00384A0Rb
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3 to 7 Working Days
  • EIF4A2 Antibody
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate<br />
 All lanes: EIF4A2 antibody at 3µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 47 kDa<br />
 Observed band size: 47 kDa<br />
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100
  • Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
$378.96 - $586.44

Description

EIF4A2 Antibody | CSB-PA00384A0Rb | Cusabio

EIF4A2 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: EIF4A2

Aliases: Eukaryotic initiation factor 4A-II (eIF-4A-II) (eIF4A-II) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-2), EIF4A2, DDX2B EIF4F

Background: Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q14240

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Eukaryotic initiation factor 4A-II protein (1-407AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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