EIF3K Antibody | CSB-PA866204ESR1HU

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SKU:
CSB-PA866204ESR1HU
Availability:
3 to 7 Working Days
  • EIF3K Antibody
  • Western blot<br />
 All lanes: EIF3K antibody at 1.72µg/ml<br />
 Lane 1: THP-1 whole cell lysate<br />
 Lane 2: HT29 whole cell lysate<br />
 Lane 3: 293T whole cell lysate<br />
 Lane 4: Hela whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/10000 dilution<br />
 Predicted band size: 26, 25 kDa<br />
 Observed band size: 26 kDa<br />
  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100
  • Immunoprecipitating EIF3K in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) <br />
 Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (20µg) <br />
£184.00 - £253.68

Description

EIF3K Antibody | CSB-PA866204ESR1HU | Cusabio

EIF3K Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: EIF3K

Aliases: Eukaryotic translation initiation factor 3 subunit K (eIF3k) (Eukaryotic translation initiation factor 3 subunit 12) (Muscle-specific gene M9 protein) (PLAC-24) (eIF-3 p25) (eIF-3 p28), EIF3K, EIF3S12

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: Q9UBQ5

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IP

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Neuroscience

Research Areas: Epigenetics & Nuclear Signaling;Neuroscience

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