DLD Antibody | CSB-PA006928LA01HU

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SKU:
CSB-PA006928LA01HU
Availability:
3 to 7 Working Days
  • DLD Antibody
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue, Rat brain tissue, Rat heart tissue, Mouse heart tissue<br />
 All lanes: DLD antibody at 2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 55, 44, 50 kDa<br />
 Observed band size: 55 kDa<br />
  • IHC image of CSB-PA006928LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
€315.80 - €488.70

Description

DLD Antibody | CSB-PA006928LA01HU | Cusabio

DLD Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: DLD

Aliases: Dihydrolipoyl dehydrogenase, mitochondrial (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (Glycine cleavage system L protein), DLD, GCSL LAD PHE3

Background: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) . In monomeric form has additional moonlighting function as serine protease (PubMed:17404228) . Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P09622

Host Species: Rabbit

Species Reactivity: Human, Rat, Mouse

Immunogen: Recombinant Human Dihydrolipoyl dehydrogenase, mitochondrial protein (398-495AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC

Tested Applications: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cancer;Metabolism;Signal transduction

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