Di-methyl-H3F3A (K79) Antibody | CSB-PA010109OA79me2HU

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CSB-PA010109OA79me2HU
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  • Di-methyl-H3F3A (K79) Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate<br />
 All lanes: H3F3A antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/40000 dilution<br />
 Predicted band size: 16 kDa<br />
 Observed band size: 16 kDa<br />
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H3F3A (CSB-PA010109OA79me2HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
£262.00 - £411.76

Description

Di-methyl-H3F3A (K79) Antibody | CSB-PA010109OA79me2HU | Cusabio

Di-methyl-H3F3A (K79) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: H3F3A

Aliases: Histone H3.3, H3F3A; H3F3B, H3.3A H3F3; H3.3B

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P84243

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Di-methyl-Lys (79) derived from Human Histone H3.3

Immunogen Species: Human

Applications: ELISA, WB, ChIP

Tested Applications: ELISA, WB, ChIP; Recommended dilution: WB:1:500-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:2000

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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