DHFR Antibody | CSB-RA264582A0HU

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CSB-RA264582A0HU
Availability:
3 to 7 Working Days
  • DHFR Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue<br />
 All lanes: DHFR antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 22, 16 kDa<br />
 Observed band size: 22 kDa<br />
  • IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-RA264582A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunoprecipitating DHFR in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA264582A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA264582A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
€373.00 - €555.00

Description

DHFR Antibody | CSB-RA264582A0HU | Cusabio

DHFR Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: DHFR

Aliases: Dihydrofolate reductase (EC 1.5.1.3), DHFR

Background: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 9B2

Uniport ID: P00374

Modified: Cancer; Cell biology; Metabolism; Signal transduction

species: Homo sapiens (Human)

Species Reactivity: Human, Mouse, Rat

Immunogen: A synthesized peptide derived from human DHFR

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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