Crotonyl-HIST1H2BC (K12) Antibody | CSB-PA010403OA12crHU

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SKU:
CSB-PA010403OA12crHU
Availability:
3 to 7 Working Days
  • Crotonyl-HIST1H2BC (K12) Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h) <br />
 All lanes: HIST1H2BC antibody at 0.97µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 14 kDa<br />
 Observed band size: 14 kDa<br />
  • Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010403OA12crHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating HIST1H2BC in 293 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA010403OA12crHU in 293 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010403OA12crHU (5µg) + 293 whole cell lysate (500µg) <br />
 Lane 3: 293 whole cell lysate (20µg) <br />
  • Chromatin Immunoprecipitation Hela (10<sup>6</sup>, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
$393.00 - $617.64

Description

Crotonyl-HIST1H2BC (K12) Antibody | CSB-PA010403OA12crHU | Cusabio

Crotonyl-HIST1H2BC (K12) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H2BC

Aliases: Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P62807

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I

Immunogen Species: Human

Applications: ELISA, WB, IF, IP, ChIP

Tested Applications: ELISA, WB, IF, IP, ChIP; Recommended dilution: WB:1:100-1:1000, IF:1:1-1:10, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: IF:1:1-1:10

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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