Crotonyl-HIST1H2AG (K125) Antibody | CSB-PA010389PA125crHU

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SKU:
CSB-PA010389PA125crHU
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  • Crotonyl-HIST1H2AG (K125) Antibody
  • Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) using CSB-PA010389PA125crHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA125crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
$393.00 - $617.64

Description

Crotonyl-HIST1H2AG (K125) Antibody | CSB-PA010389PA125crHU | Cusabio

Crotonyl-HIST1H2AG (K125) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H2AG

Aliases: Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM, H2AFP; H2AFC; H2AFD; H2AFI; H2AFN

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P0C0S8

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Crotonyl-Lys (125) derived from Human Histone H2A type 1

Immunogen Species: Human

Applications: ELISA, IF, ChIP

Tested Applications: ELISA, IF, ChIP; Recommended dilution: IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: IF:1:50-1:200

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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