CDK4 Antibody | CSB-RA965615A0HU

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SKU:
CSB-RA965615A0HU
Availability:
3 to 7 Working Days
  • CDK4 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate<br />
 All lanes: CDK4 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 34, 21 kDa<br />
 Observed band size: 34 kDa<br />
  • Immunoprecipitating CDK4 in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA965615A0HU in Hela whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)<br />
 Lane 2:CSB-RA965615A0HU(2µg)+ Hela whole cell lysate(500µg)<br />
 Lane 3: Hela whole cell lysate (10µg)<br />
£298.40 - £444.00

Description

CDK4 Antibody | CSB-RA965615A0HU | Cusabio

CDK4 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: CDK4

Aliases: Cyclin-dependent kinase 4 (EC 2.7.11.22) (Cell division protein kinase 4) (PSK-J3), CDK4

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 8F2

Uniport ID: P11802

Modified: Isotype/Loading Controls; Epigenetics and Nuclear Signaling; Cancer; Cell biology

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human CDK4

Tested Applications: ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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