CDH1 Monoclonal Antibody | CSB-MA005034A0m

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CSB-MA005034A0m
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3 to 7 Working Days
  • CDH1 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: Caco205 whole cell lysate<br />
 All lanes: CDH1 antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 98, 91 kDa<br />
 Observed band size: 110, 120 kDa<br />
  • Immunofluorescence staining of Hela cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of MCF-7 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of SW620 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing MCF-7 cells stained with CSB-MA005034A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00

Description

CDH1 Monoclonal Antibody | CSB-MA005034A0m | Cusabio

CDH1 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Monoclonal Antibody

Target Name: CDH1

Aliases: Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3], CDH1, CDHE UVO

Relevance: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333) . They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333) . Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 9H2D3

Uniport ID: P12830

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human, Mouse

Immunogen: Recombinant Human Cadherin-1 protein (155-707AA)

Immunogen Species: Human

Applications: ELISA, WB, IF, FC

Tested Applications: ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:500-1:5000

Dilution Ratio2: IF:1:50-1:200

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cancer;Developmental biology;Signal transduction

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