CD80 Antibody | CSB-RA246383A0HU

(No reviews yet) Write a Review
3 to 7 Working Days
  • CD80 Antibody
  • Western Blot<br />
 Positive WB detected in: Raji whole cell lysate<br />
 All lanes: CD80 antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 34, 30, 19 kDa<br />
 Observed band size: 60 kDa<br />
  • IHC image of CSB-RA246383A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Immunoprecipitating CD80 in Raji whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-RA246383A0HU in Raji whole cell lysate.
 For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)<br />
 Lane 2: CSB-RA246383A0HU(2µg)+ Raji whole cell lysate(500µg)<br />
 Lane 3: Raji whole cell lysate (10µg)<br />
€373.00 - €555.00


CD80 Antibody | CSB-RA246383A0HU | Cusabio

CD80 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to

Antibody Type: Recombinant

Target Names: CD80

Aliases: T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80), CD80, CD28LG CD28LG1 LAB7

Background: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 3C3

Uniport ID: P33681

Modified: Immunology; Stem cells

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human CD80

Tested Applications: ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

View AllClose

0 Reviews

View AllClose