CD44 Monoclonal Antibody | CSB-MA004938A0m

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CSB-MA004938A0m
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  • CD44 Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate<br />
 All lanes: CD44 antibody at 1:1500<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54, 47, 40, 44, 33, 74, 76, 38, 16 kDa<br />
 Observed band size: 95 kDa<br />
  • Western Blot<br />
 Positive WB detected in: A549 whole cell lysate<br />
 All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000<br />
 Secondary<br />
 Goat polyclonal to Mouse IgG at 1/10000 dilution<br />
 Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54, 47, 40, 44, 33, 74, 76, 38, 16 kDa<br />
 Observed band size: 95 kDa<br />
  • IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of MCF-7 cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing Hela cells stained with CSB-MA004938A0m (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00

Description

CD44 Monoclonal Antibody | CSB-MA004938A0m | Cusabio

CD44 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: CD44

Aliases: CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4

Relevance: Receptor for hyaluronic acid (HA) . Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity) .

Isotype: IgG1, Igk

Conjugate: Non-conjugated

Clone Number: 6G2D2

Uniport ID: P16070

Alternatives To SCBT: #N/A

Host Species: Mouse

Species Reactivity: Human

Immunogen: Recombinant Human CD44 antigen protein (21-220AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:1000-1:40000, IHC:1:50-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: WB:1:1000-1:40000

Dilution Ratio2: IHC:1:50-1:200

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology

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