CD14 Monoclonal Antibody | CSB-MA004879A0m

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SKU:
CSB-MA004879A0m
Availability:
3 to 7 Working Days
  • CD14 Monoclonal Antibody
  • Western Blot<br /> Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue<br /> All lanes: CD14 antibody at 1:2500<br /> Secondary<br /> Goat polyclonal to Mouse IgG at 1/10000 dilution<br /> Predicted band size: 41 kDa<br /> Observed band size: 55 kDa<br />
  • Western Blot<br /> Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate<br /> All lanes: CD14 antibody at 1:1800<br /> Secondary<br /> Goat polyclonal to Mouse IgG at 1/10000 dilution<br /> Predicted band size: 41 kDa<br /> Observed band size: 55 kDa<br />
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of A549 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of Hela cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of HepG2 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of RAW264.7 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Immunofluorescence staining of SY5Y cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L) .
  • Overlay histogram showing Jurkat cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
  • Overlay histogram showing RAW264.7 cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.
€373.00 - €555.00

Description

CD14 Monoclonal Antibody | CSB-MA004879A0m | Cusabio

CD14 Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Monoclonal Antibody

Target Name: CD14

Aliases: Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form], CD14

Relevance: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655) . In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135) . Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211) . Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187) .

Isotype: IgG1

Conjugate: Non-conjugated

Clone Number: 14G1F3

Uniport ID: P08571

Alternatives To SCBT: sc-58951/sc-19588/sc-7328/sc-52457/sc-1182

Host Species: Mouse

Species Reactivity: Human, Mouse, Rabbit

Immunogen: Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, FC

Tested Applications: ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200

Purification Method: >95%, Protein A purified

Dilution Ratio1: WB:1:1000-1:5000

Dilution Ratio2: IHC:1:50-1:200

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Immunology

Research Areas: Immunology;Stem cells

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