CASP7 Antibody | CSB-PA15479A0Rb

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CSB-PA15479A0Rb
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  • CASP7 Antibody
  • Western blot<br />
 All lanes: CASP7 antibody at 2µg/ml<br />
 Lane 1: A549 whole cell lysate<br />
 Lane 2: HGC-27 whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/10000 dilution<br />
 Predicted band size: 35, 29, 38, 32 kDa<br />
 Observed band size: 35 kDa<br />
  • IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA15479A0Rb diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of MCF-7 cells with CSB-PA15479A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating CASP7 in HEK293 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA15479A0Rb in HEK293 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA15479A0Rb (8µg) + HEK293 whole cell lysate (500µg) <br />
 Lane 3: HEK293 whole cell lysate (10µg) <br />
$378.96 - $586.44

Description

CASP7 Antibody | CSB-PA15479A0Rb | Cusabio

CASP7 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: CASP7

Aliases: Caspase-7 (CASP-7) (EC 3.4.22.60) (Apoptotic protease Mch-3) (CMH-1) (ICE-like apoptotic protease 3) (ICE-LAP3) [Cleaved into: Caspase-7 subunit p20; Caspase-7 subunit p11], CASP7, MCH3

Background:

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P55210

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Caspase-7 protein (24-198AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:200-1:500

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cancer;Cell biology;Metabolism

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