C3 Antibody | CSB-RA303909A0HU

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SKU:
CSB-RA303909A0HU
Availability:
3 to 7 Working Days
  • C3 Antibody
  • Western Blot<br />
 Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate<br />
 All lanes: C3 antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 188 kDa<br />
 Observed band size: 115 kDa<br />
  • IHC image of CSB-RA303909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • Overlay histogram showing HepG2 cells stained with CSB-RA303909A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
$447.60 - $666.00

Description

C3 Antibody | CSB-RA303909A0HU | Cusabio

C3 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Antibody Type: Recombinant

Target Names: C3

Aliases: Complement C3 (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) [Cleaved into: Complement C3 beta chain, C3-beta-c (C3bc), Complement C3 alpha chain, C3a anaphylatoxin, Acylation stimulating protein (ASP) (C3adesArg), Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3dg fragment, Complement C3g fragment, Complement C3d fragment, Complement C3f fragment, Complement C3c alpha' chain fragment 2], C3, CPAMD1

Background: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 4D12

Uniport ID: P01024

Modified: Neuroscience; Immunology

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human C3

Tested Applications: ELISA, WB, IHC, FC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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