Butyrly-HIST1H3A (K23) Antibody | CSB-PA010418OA23butHU

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CSB-PA010418OA23butHU
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  • Butyrly-HIST1H3A (K23) Antibody
  • Western Blot<br />
 Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br />
 All lanes: HIST1H3A antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/40000 dilution<br />
 Predicted band size: 16 kDa<br />
 Observed band size: 16 kDa<br />
  • Immunocytochemistry analysis of CSB-PA010418OA23butHU diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010418OA23butHU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating HIST1H3A in Hela whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA010418OA23butHU in Hela whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010418OA23butHU (5µg) + Hela whole cell lysate (500µg) <br />
 Lane 3: Hela whole cell lysate (20µg) <br />
  • Chromatin Immunoprecipitation Hela (10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA23butHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
$393.00 - $617.64

Description

Butyrly-HIST1H3A (K23) Antibody | CSB-PA010418OA23butHU | Cusabio

Butyrly-HIST1H3A (K23) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H3A

Aliases: Histone H3.1 (Histone H3/a) (Histone H3/b) (Histone H3/c) (Histone H3/d) (Histone H3/f) (Histone H3/h) (Histone H3/i) (Histone H3/j) (Histone H3/k) (Histone H3/l), HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J, H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P68431

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Butyrly-Lys (23) derived from Human Histone H3.1

Immunogen Species: Human

Applications: ELISA, WB, ICC, IF, IP, ChIP

Tested Applications: ELISA, WB, ICC, IF, IP, ChIP; Recommended dilution: WB:1:1000-1:5000, ICC:1:10-1:100, IF:1:10-1:100, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: ICC:1:10-1:100

Dilution Ratio4: IF:1:10-1:100

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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