BUB1B Antibody | CSB-RA263021A0HU

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SKU:
CSB-RA263021A0HU
Availability:
3 to 7 Working Days
  • BUB1B Antibody
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate<br />
 All lanes: BubR1 antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 120, 106, 122 kDa<br />
 Observed band size: 125 kDa<br />
  • Overlay histogram showing Hela cells stained with CSB-RA263021A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
$447.60 - $666.00

Description

BUB1B Antibody | CSB-RA263021A0HU | Cusabio

BUB1B Antibody is Available at Gentaur Genprice with the fastest delivery.

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Antibody Type: Recombinant

Target Names: BUB1B

Aliases: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (EC 2.7.11.1) (MAD3/BUB1-related protein kinase) (hBUBR1) (Mitotic checkpoint kinase MAD3L) (Protein SSK1), BUB1B, BUBR1 MAD3L SSK1

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Isotype: Rabbit IgG

Conjugate: Non-conjugated

Clonality: Monoclonal

Clone Number: 7H4

Uniport ID: O60566

Modified: Epigenetics and Nuclear Signaling; Cancer; Cell biology

species: Homo sapiens (Human)

Species Reactivity: Human

Immunogen: A synthesized peptide derived from human BubR1

Tested Applications: ELISA, WB, FC; Recommended dilution: WB:1:500-1:5000, FC:1:20-1:200

Purification Method: Affinity-chromatography

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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