BMPR1A Antibody | CSB-PA002748LA01HU

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CSB-PA002748LA01HU
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  • BMPR1A Antibody
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002748LA01HU at dilution of 1:100
  • Western Blot<br />
 Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate<br />
 All lanes: BMPR1A antibody at 3.3µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 61 kDa<br />
 Observed band size: 61 kDa<br />
  • IHC image of CSB-PA002748LA01HU diluted at 1:1000 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of HepG2 cells with CSB-PA002748LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
$378.96 - $586.44

Description

BMPR1A Antibody | CSB-PA002748LA01HU | Cusabio

BMPR1A Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: BMPR1A

Aliases: Bone morphogenetic protein receptor type-1A (BMP type-1A receptor) (BMPR-1A) (EC 2.7.11.30) (Activin receptor-like kinase 3) (ALK-3) (Serine/threonine-protein kinase receptor R5) (SKR5) (CD antigen CD292), BMPR1A, ACVRLK3 ALK3

Background: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP-2 and BMP-4. Positively regulates chondrocyte differentiation through GDF5 interaction.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P36894

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Bone morphogenetic protein receptor type-1A protein (177-532AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:200-1:500

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:5000

Dilution Ratio3: IHC:1:100-1:1000

Dilution Ratio4: IF:1:200-1:500

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Cancer;Cardiovascular;Metabolism;Signal transduction;Stem cells

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