BCL6 (Ab-333) Antibody | CSB-PA935424

BCL6 (Ab-333) Antibody | CSB-PA935424 | Cusabio

BCL6 (Ab-333) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: BCL6

Aliases: B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein homolog; Bcl-5; Bcl5

Background: Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T (FH) cells), promotes the expression of T (FH) -related genes but inhibits the differentiation of T (H) 1, T (H) 2 and T (H) 17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Kerckaert J.-P., Nat. Genet. 5:66-70 (1993) .
Ye B.H., Science 262:747-750 (1993) .
Miki T., Blood 83:26-32 (1994) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P41182

Host Species: Rabbit

Species Reactivity: Human, Mouse

Immunogen: Synthesized non-phosphopeptide derived from human Bcl-6 around the phosphorylation site of serine 333 (P-Q-S (p) -P-Q) .

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:3000

Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:3000

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Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling;Cancer