BCAP31 Antibody | CSB-PA03425A0Rb

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SKU:
CSB-PA03425A0Rb
Availability:
3 to 7 Working Days
  • BCAP31 Antibody
  • Western Blot<br />
 Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, Rat lung tissue, Rat brain tissue, Mouse lung tissue, Mouse brain tissue<br />
 All lanes: BCAP31 antibody at 4.8µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 28, 35 kDa<br />
 Observed band size: 28 kDa<br />
  • IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-PA03425A0Rb diluted at 1:1200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells with CSB-PA03425A0Rb at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunoprecipitating BCAP31 in Mouse liver tissue<br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA03425A0Rb in Mouse liver tissue.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA03425A0Rb (6µg) + Mouse liver tissue (500µg) <br />
 Lane 3: Mouse liver tissue (10µg) <br />
£252.64 - £390.96

Description

BCAP31 Antibody | CSB-PA03425A0Rb | Cusabio

BCAP31 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: BCAP31

Aliases: B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (6C6-AG tumor-associated antigen) (Protein CDM) (p28), BCAP31, BAP31 DXS1357E

Background: Functions as a chaperone protein. Is one of the most abundant endoplasmic reticulum (ER) proteins. Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) . Also serves as a cargo receptor for the export of transmembrane proteins. May be involved in CASP8-mediated apoptosis.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P51572

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human B-cell receptor-associated protein 31 protein (2-243AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:1000-1:2000

Dilution Ratio4: IF:1:200-1:500

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cell biology

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