ATP6V1G1 Antibody | CSB-PA00135A0Rb

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SKU:
CSB-PA00135A0Rb
Availability:
3 to 7 Working Days
  • ATP6V1G1 Antibody
  • Western blot<br />
 All lanes: V-type proton ATPase subunit G 1 antibody at 2µg/ml + 293T whole cell lysate<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/15000 dilution<br />
 Predicted band size: 13 kDa<br />
 Observed band size: 13 kDa<br />
  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00135A0Rb at dilution of 1:50
  • Immunofluorescence staining of PC-3 cells with CSB-PA00135A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00135A0Rb at dilution of 1:100
€315.80 - €488.70

Description

ATP6V1G1 Antibody | CSB-PA00135A0Rb | Cusabio

ATP6V1G1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: ATP6V1G1

Aliases: V-type proton ATPase subunit G 1 (V-ATPase subunit G 1) (V-ATPase 13 kDa subunit 1) (Vacuolar proton pump subunit G 1) (Vacuolar proton pump subunit M16), ATP6V1G1, ATP6G ATP6G1 ATP6J

Background: This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O75348

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction

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