ATP5A1 Antibody | CSB-PA057763

ATP5A1 Antibody | CSB-PA057763 | Cusabio

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Product Type: Polyclonal Antibody

Target Names: ATP5A1

Aliases: ATP synthase alpha chain; mitochondrial precursor; ATP5A; ATPA; EC 3.6.3.14

Background: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites By similarity.

Kataoka H., Biochim. Biophys. Acta 1089:393-395 (1991) .
Godbout R., Gene 123:195-201 (1993) .
Akiyama S., Biochim. Biophys. Acta 1219:129-140 (1994)

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P25705

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Synthesized peptide derived from internal of human ATP5A1.

Immunogen Species: Human

Applications: ELISA, WB

Tested Applications: ELISA, WB;WB:1:500-1:3000

Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:3000

Dilution Ratio3:

Dilution Ratio4:

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Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Tags & Cell Markers;Metabolism;Signal transduction