APAF1 Antibody | CSB-PA109273

APAF1 Antibody | CSB-PA109273 | Cusabio

APAF1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: APAF1

Aliases: APAF1-interacting protein; APIP; APIP2; CGI-29 protein; MMRP19

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Keigo Machida, J. Biol. Chem., Apr 2001; 276: 12140 - 12146.
Liam O 鐤宱nnor, Cancer Res., Mar 2000; 60: 1217 - 1220.
Zeping Wang, Nucleic Acids Res., Apr 2005; 33: 2248 - 2258
Govindaraj Anumanthan, Cancer Res., Nov 2006; 66: 10824 - 10832.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: O14727

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Synthesized peptide derived from human APAF-1-ALT.

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF

Tested Applications: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200

Purification Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:500-1:3000

Dilution Ratio3: IHC:1:50-1:200

Dilution Ratio4: IF:1:100-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form: liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Cell Biology

Research Areas: Cancer;Cell biology;Metabolism