ANXA1 Antibody | CSB-PA13457A0Rb

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SKU:
CSB-PA13457A0Rb
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3 to 7 Working Days
  • ANXA1 Antibody
  • Western Blot<br />
 Positive WB detected in: PC-3 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate<br />
 All lanes: ANXA1 antibody at 2µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 39 kDa<br />
 Observed band size: 39 kDa<br />
  • Immunoprecipitating ANXA1 in K562 whole cell lysate<br />
 Lane 1: Rabbit control IgG instead of CSB-PA13457A0Rb in K562 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA13457A0Rb (8µg) + K562 whole cell lysate (500µg) <br />
 Lane 3: K562 whole cell lysate (10µg) <br />
  • Immunofluorescence staining of Hela cells with CSB-PA13457A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
£252.64 - £390.96

Description

ANXA1 Antibody | CSB-PA13457A0Rb | Cusabio

ANXA1 Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: ANXA1

Aliases: Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35), ANXA1, ANX1 LPC1

Background: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P04083

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Recombinant Human Annexin A1 protein (5-346AA)

Immunogen Species: Human

Applications: ELISA, WB, IF, IP

Tested Applications: ELISA, WB, IF, IP; Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4: IP:1:200-1:2000

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Signal Transduction

Research Areas: Signal transduction

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