Acetyl-HIST1H2AG (K74) Antibody | CSB-PA010389OA74acHU

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SKU:
CSB-PA010389OA74acHU
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3 to 7 Working Days
  • Acetyl-HIST1H2AG (K74) Antibody
  • Western Blot<br />
 Detected samples: Hela whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br />
 All lanes: HIST1H2AG antibody at 1:100<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 15 kDa<br />
 Observed band size: 15 kDa<br />
  • Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010389OA74acHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Immunocytochemistry analysis of CSB-PA010389OA74acHU diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
$393.00 - $617.64

Description

Acetyl-HIST1H2AG (K74) Antibody | CSB-PA010389OA74acHU | Cusabio

Acetyl-HIST1H2AG (K74) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H2AG

Aliases: Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM, H2AFP; H2AFC; H2AFD; H2AFI; H2AFN

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P0C0S8

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Acetyl-Lys (74) derived from Human Histone H2A type 1

Immunogen Species: Human

Applications: ELISA, WB, ICC, IF

Tested Applications: ELISA, WB, ICC, IF; Recommended dilution: WB:1:100-1:1000, ICC:1:1-1:10, IF:1:1-1:10

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: ICC:1:1-1:10

Dilution Ratio4: IF:1:1-1:10

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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