Acetyl-HIST1H1C (K96) Antibody | CSB-PA010378PA96acHU

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SKU:
CSB-PA010378PA96acHU
Availability:
3 to 7 Working Days
  • Acetyl-HIST1H1C (K96) Antibody
  • Western Blot<br />
 Detected samples: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br />
 All lanes: HIST1H1C antibody at 1:2000<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/40000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • Immunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100
  • Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) <br />
 Lane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) .
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA010378PA96acHU (5µg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg) <br />
 Lane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg) <br />
$393.00 - $617.64

Description

Acetyl-HIST1H1C (K96) Antibody | CSB-PA010378PA96acHU | Cusabio

Acetyl-HIST1H1C (K96) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H1C

Aliases: Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16403

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Acetyl-Lys (96) derived from Human Histone H1.2

Immunogen Species: Human

Applications: ELISA, WB, ICC, IF, IP, ChIP

Tested Applications: ELISA, WB, ICC, IF, IP, ChIP; Recommended dilution: WB:1:1000-1:5000, ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: ICC:1:20-1:200

Dilution Ratio4: IF:1:50-1:200

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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