Acetyl-H2AFZ (K11) Antibody | CSB-PA010100PA11acHU

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SKU:
CSB-PA010100PA11acHU
Availability:
3 to 7 Working Days
  • Acetyl-H2AFZ (K11) Antibody
  • Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010100PA11acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-H2AFZ (CSB-PA010100PA11acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
  • Western Blot<br />
 Positive WB detected in: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) <br />
 All lanes: H2AFZ antibody at 1µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 14 kDa<br />
 Observed band size: 14 kDa<br />
$393.00 - $617.64

Description

Acetyl-H2AFZ (K11) Antibody | CSB-PA010100PA11acHU | Cusabio

Acetyl-H2AFZ (K11) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: H2AFZ

Aliases: Histone H2A.Z (H2A/z), H2AFZ, H2AZ

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P0C0S5

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of Acetyl-Lys (11) derived from Human Histone H2A.Z

Immunogen Species: Human

Applications: ELISA, WB, IF, ChIP

Tested Applications: ELISA, WB, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:200-1:2000

Dilution Ratio3: IF:1:50-1:200

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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