ACAA2 Antibody | CSB-PA001118LA01HU

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SKU:
CSB-PA001118LA01HU
Availability:
3 to 7 Working Days
  • ACAA2 Antibody
  • Western Blot<br />
 Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat lung tissue, Mouse liver tissue, Mouse lung tissue<br />
 All lanes: ACAA2 antibody at 2.4µg/ml<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 42 kDa<br />
 Observed band size: 42 kDa<br />
  • IHC image of CSB-PA001118LA01HU diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescent analysis of HepG2 cells using CSB-PA001118LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L)
  • Immunoprecipitating ACAA2 in A549 whole cell lysate <br />
 Lane 1: Rabbit control IgG (1µg) instead of CSB-PA001118LA01HU in A549 whole cell lysate.
 For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) <br />
 Lane 2: CSB-PA001118LA01HU (6µg) + A549 whole cell lysate (500µg) <br />
 Lane 3: A549 whole cell lysate (10µg) <br />
€315.80 - €488.70

Description

ACAA2 Antibody | CSB-PA001118LA01HU | Cusabio

ACAA2 Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: ACAA2

Aliases: 3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1), ACAA2

Background: Abolishes BNIP3-mediated apoptosis and mitochondrial damage.

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P42765

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Immunogen: Recombinant Human 3-ketoacyl-CoA thiolase, mitochondrial protein (188-257AA)

Immunogen Species: Human

Applications: ELISA, WB, IHC, IF, IP

Tested Applications: ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:500, IP:1:200-1:2000

Purification Method: >95%, Protein G purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:1000-1:5000

Dilution Ratio3: IHC:1:500-1:1000

Dilution Ratio4: IF:1:50-1:500

Dilution Ratio5: IP:1:200-1:2000

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Tags & Cell Markers

Research Areas: Cardiovascular;Tags & Cell Markers;Metabolism;Signal transduction

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