6*His Monoclonal Antibody | CSB-MA000011M0m

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SKU:
CSB-MA000011M0m
Availability:
3 to 7 Working Days
  • 6*His Monoclonal Antibody
  • Western Blot<br />
 Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng<br />
 All lanes: HIS antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 1/50000 dilution<br />
 Predicted band size: 55 kDa<br />
 Observed band size: 55 kDa<br />
  • Western Blot<br />
 Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng<br />
 All lanes: HIS antibody at 1:1000<br />
 Secondary<br />
 Goat polyclonal to mouse IgG at 1/50000 dilution<br />
 Predicted band size: 60 kDa<br />
 Observed band size: 60 kDa<br />
€256.00 - €360.00
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Description

6*His Monoclonal Antibody | CSB-MA000011M0m | Cusabio

6*His Monoclonal Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Tag/Control Antibodies

Target Name: 6*His

Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

Relevance: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

Isotype: IgG2a

Conjugate: Non-conjugated

Clone Number: 3G5H8

Host Species: Mouse

Species Reactivity: All

Immunogen: HHHHHH(6x His) synthetic peptide conjugate to KLH

Applications: ELISA, WB

Tested Applications: ELISA, WB; Recommended dilution: WB:1:500-1:2000

Purification Method: >95%, Protein A purified

Dilution Ratio1: WB:1:500-1:2000

Dilution Ratio2:

Dilution Ratio3:

Dilution Ratio4:

Dilution Ratio5:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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