2-hydroxyisobutyryl-HIST1H1C (K158) Antibody | CSB-PA010378OA158hibHU

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SKU:
CSB-PA010378OA158hibHU
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  • 2-hydroxyisobutyryl-HIST1H1C (K158) Antibody
  • Western Blot<br />
 Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br />
 All lanes: HIST1H1C antibody at 1:100<br />
 Secondary<br />
 Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
 Predicted band size: 22 kDa<br />
 Observed band size: 22 kDa<br />
  • Immunocytochemistry analysis of CSB-PA010378OA158hibHU diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Chromatin Immunoprecipitation Hela (10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA158hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
£262.00 - £411.76

Description

2-hydroxyisobutyryl-HIST1H1C (K158) Antibody | CSB-PA010378OA158hibHU | Cusabio

2-hydroxyisobutyryl-HIST1H1C (K158) Antibody is Available at Gentaur Genprice with the fastest delivery.

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Product Type: Polyclonal Antibody

Target Names: HIST1H1C

Aliases: Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16403

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of 2-hydroxyisobutyryl-Lys (158) derived from Human Histone H1.2

Immunogen Species: Human

Applications: ELISA, WB, ICC, ChIP

Tested Applications: ELISA, WB, ICC, ChIP; Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: ICC:1:10-1:100

Dilution Ratio4:

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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