2-hydroxyisobutyryl-HIST1H1C (K109) Antibody | CSB-PA010378OA109hibHU

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SKU:
CSB-PA010378OA109hibHU
Availability:
3 to 7 Working Days
  • 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody
  • Western Blot<br /> Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h<br /> All lanes: HIST1H1C antibody at 3.5µg/ml<br /> Secondary<br /> Goat polyclonal to rabbit IgG at 1/50000 dilution<br /> Predicted band size: 22 kDa<br /> Observed band size: 22 kDa<br />
  • Immunocytochemistry analysis of CSB-PA010378OA109hibHU diluted at 1:50 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010378OA109hibHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
  • Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA109hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
£262.00 - £411.76

Description

2-hydroxyisobutyryl-HIST1H1C (K109) Antibody | CSB-PA010378OA109hibHU | Cusabio

2-hydroxyisobutyryl-HIST1H1C (K109) Antibody is Available at Gentaur Genprice with the fastest delivery.

Online Order Payment is possible or send quotation to info@gentaur.com.

Product Type: Polyclonal Antibody

Target Names: HIST1H1C

Aliases: Histone H1.2, Histone H1c, Histone H1d, Histone H1s-1, HIST1H1C, H1F2

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity) .

Isotype: IgG

Conjugate: Non-conjugated

Clonality: Polyclonal

Uniport ID: P16403

Host Species: Rabbit

Species Reactivity: Human

Immunogen: Peptide sequence around site of 2-hydroxyisobutyryl-Lys (109) derived from Human Histone H1.2

Immunogen Species: Human

Applications: ELISA, WB, ICC, IF, ChIP

Tested Applications: ELISA, WB, ICC, IF, ChIP; Recommended dilution: WB:1:100-1:1000, ICC:1:20-1:200, IF:1:20-1:200

Purification Method: Antigen Affinity Purified

Dilution Ratio1: ELISA:1:2000-1:10000

Dilution Ratio2: WB:1:100-1:1000

Dilution Ratio3: ICC:1:20-1:200

Dilution Ratio4: IF:1:20-1:200

Dilution Ratio5:

Dilution Ratio6:

Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form: Liquid

Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Initial Research Areas: Epigenetics and Nuclear Signaling

Research Areas: Epigenetics & Nuclear Signaling

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Formyl-HIST1H1C (K109) Antibody | CSB-PA010378OA109forHU | CusabioFormyl-HIST1H1C (K109) Antibody is Available at Gentaur Genprice with the fastest delivery.Online Order Payment is possible or send...
SKU:
CSB-PA010378OA109forHU
Availability:
3 to 7 Working Days